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. 2007 Jun 27;405(Pt 2):269–276. doi: 10.1042/BJ20061762

Figure 2. Knockdown of PLC-γ1 in H4IIE cells by a specific siRNA.

Figure 2

(A) Immunofluorescence microscopy of PLC-γ1 (green) in cells transfected for 96 h with negative control siRNA (first row) and PLC-γ1-01 siRNA (second row). Second column shows staining of the cells nuclei with DAPI (blue), and third column shows merged images. Results are representative of four individual experiments. Bar=20 μm. (B) Reduction of the PLC-γ1 protein as determined by Western blotting. Expression of GAPDH served as a loading control. Molecular mass markers are indicated on the left-hand side of the Figure. Results are representative of four individual experiments.