Figure 4. Effect of PLC-γ1 knockdown on I_SOC in H4IIE cells.

(A) Development of I_SOC in response to intracellular IP₃ in H4IIE cells treated with either control siRNA or PLC-γ1-01 siRNA for 72 and 96 h. (B) Scatter plot of the data presented in panel (A) and the data obtained on non-transfected cells and cells treated with transfection reagent only at 90 s after establishing whole cell. The P values are relative to the Control (cells transfected with control siRNA). (C) Activation of I_SOC by intracellular application of thapsigargin in H4IIE cells treated with either control siRNA or PLC-γ1-01 siRNA for 96 h.