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. 2007 Jun 27;405(Pt 2):331–340. doi: 10.1042/BJ20070299

Figure 1. Translocation of endogenous PKCs to the particulate fraction by ATP-competitive inhibitors.

Figure 1

(A) HeLa cells were treated with 1 or 10 μM GF 109203X for 20 min. (B) HeLa cells were treated with 5 μM GF 109203X for the indicated times. (C) HeLa cells were treated with 0.1 μM staurosporine or 5 μM Ro-31-8220 for 30 min. (D) HeLa cells were treated with the indicated concentrations of GF 109203X or Gö 6976 for 15 min. Subcellular fractions were obtained as described in the Experimental section and subjected to SDS/PAGE, followed by immunoblotting with anti-PKCα, anti-PKCδ or anti-PKCζ and with anti-actin antibody. Actin was used as a loading control. Results are representative of at least three separate experiments.