Skip to main content
. 2007 Jun 27;405(Pt 2):331–340. doi: 10.1042/BJ20070299

Figure 2. ATP-competitive inhibitors selectively induce membrane redistribution of DAG-sensitive PKCs.

Figure 2

(A) HeLa cells transiently expressing PKCα–, PKCδ– or PKCζ–GFP were treated with GF 109203X at 5 μM for 60 min, 1 μM for 30 min or 10 μM for 60 min respectively. (B) Percentage of cells displaying GF 109203X-induced membrane redistribution of PKC. HeLa cells transiently expressing GFP-tagged cPKCs (PKCα and βII), nPKCs (PKCδ and ϵ), aPKC (PKCζ) were treated with GF 109203X at the indicated concentrations for 60, 15 or 60 min respectively. A total of 10–14 cells were observed. (C) HeLa cells transiently expressing PKCα- or PKCδ–GFP were treated with GF 109203X at 5 or 1 μM respectively for the indicated times.