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. 2007 Jun 27;405(Pt 2):233–241. doi: 10.1042/BJ20061867

Figure 6. Grp94−/− cells are hypersensitive to calcium perturbation.

Figure 6

(A) Hypersensitivity to Tg treatment. Wild-type (●) and grp94−/− (□) ES cells were treated with the indicated concentrations of Tg in triplicate wells and cell viability was assessed after 7 h using an XTT colorimetric assay. (B) Proliferation of wild-type (WT) ES cells when grown in the presence or absence of 1 mM EGTA in the medium. The number of viable cells was counted and cells were evaluated over several doubling times. (C) Proliferation of grp94−/− ES cells when grown in the presence or absence of 1 mM EGTA in the medium. KO, knockout.