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. 2007 Jun 27;405(Pt 2):251–259. doi: 10.1042/BJ20061846

Figure 3. TGF-β up-regulates nox4 mRNA transcription and Rac1 protein level.

Figure 3

Hepatocytes were treated with TGF-β (1 ng/ml) at the indicated times. (A) RT-PCR analysis was performed to analyse the expression levels of nox1, nox2 and nox4. (B) Rac1 protein levels were analysed by Western blotting. (C) RT-PCR was performed to analyse rac1 transcript levels. (D) The effect of the TβR-I inhibitor SB431542 (10 μM) on TGF-β-mediated increase in nox4 transcription and Rac1 protein level (3 h treatment with 1 ng/ml TGF-β). In all cases, a representative experiment of at least three is shown.