Fig. 4.

Mycobacterium leprae sonicate (Mls) alters the expression of B7-1, CD28, intercellular adhesion molecule-1 (ICAM-1) and LFA-1α on the lymphocytes of healthy volunteers. The lymphocytes were cultured with Mls or purified protein derivative (PPD) for 48 h and labelled with the MoAbs against the costimulatory molecules for FACScan. Staining was done as described in the legend to Fig. 3. Data are presented as percentage difference in mean fluorescence intensity (MFI) of the expression of costimulatory molecules compared with the lymphocytes cultured in the absence of Mls or PPD (mean ± s.d.; n = 7).