Fig. 4.
Synovial macrophage intracellular lipocortin 1. Lipocortin 1 protein was detected in cultured adjuvant arthritis (AA) synovial (a,b) and peritoneal (c,d) macrophages by permeabilization flow cytometry using a specific anti-lipocortin 1 MoAb. Data are from a single experiment representative of four experiments, and show fluorescence intensity on the abscissa (log scale) and relative cell number on the ordinate. (a) After 3 h of treatment with dexamethasone 10−7m (—), lipocortin 1 fluorescence was no different with control treatment (—). (b) After 24 h of treatment, dexamethasone was associated with increased intracellular lipocortin 1. Similar results were obtained in peritoneal macrophages studied for (c) 3 h and (d) 24 h.