Fig. 2.

Cytolytic activity of bulk splenic mononuclear cells from BALB/c mice immunized with 2mg each of pCMV160IIIB and pcREV (total 4μg) in a mixture formulated with the indicated dose of Ubenimex (UBX). Effector spleen cells were cultured with the V3 peptide (CTL epitope of HIV-1_IIIB), and syngenic cells (P815; H-2^d) pulsed with or without the same peptide were used as targets. Specific cytolytic activity was titrated with an effector-to-target cell (E/T) ratio of as 5, 20 and 80, but antigen-unrestricted cytolysis was assayed at E/T ratio 80 only. Duplicated assays were performed and results are expressed as the means±s.e.m. of three or five mice comprising the control or experimental group, respectively. *, ** Significant differences between the UBX-formulated and unformulated groups (P <0.05 and P <0.01, respectively).