Fig. 1.
Selected, silver-stained two-dimensional electrophoresic (2-D) gels containing hydrophobic interaction chromatography (HIC) extracted proteins from acute-phase mouse serum. Immobiline DryStrips (18 cm) pH 4–7 (a–c) and 3–9 (d), ExcelGel SDS 8–18 and low molecular weight markers (Pharmacia) were used. Spots of interest are indicated by arrowheads and numbers. The sequence found in spot 5 was partially in accordance with mouse apoA2, and the protein in spot 6 could not be revealed by the presented techniques. The relative focusing positions and partial amino acid sequences of spots 1–3 are in accordance with SAA1 (spot 1), SAA2 (spot 2) and SAASJL/J pI 5.9 (spot 3). Spot 4 represents an SAA2- or SAASJL/J pI 5.9-like protein. In spots 2 and 3 small amounts of SAA1-like sequence could also be detected. (a) Control group. (b) Group 1. (c) Group 6. (d) Group 4. In the latter group, the five spots which are located on the basic side of the SAA spots (corresponding to pH 7–8) probably represent haemoglobin [32].