Fig. 3.

Analysis of T lymphocyte populations in the lung following reactivation of latent Mycobacterium tuberculosis infection. Mice with latent infection were restrained to activate the hypothalamic–pituitary–adrenal (HPA) axis. Immediately following HPA activation and at the times indicated the T lymphocyte populations in the lung were analysed using multi-parameter flow cytometry. Mice with latent disease served as controls. No differences in the cell populations from mice with latent disease were observed throughout the time course. The data are therefore collapsed and expressed as time 0, i.e. before HPA activation. The number of cells in each subpopulation was calculated as described in the legend for Fig. 2. (a) CD45^hi/CD44^lo. (b) CD45^lo/CD44^lo. (c) CD45^lo/CD44^hi. Values represent means ± s.e.m. for four groups of n = 3 mice. The effect of HPA activation and of time was significant on the naive, memory and activated populations as determined by anova. □, CD4; ▪, CD8.