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High concentrations of recombinant Fab are required to compete for MoAb 47 [2] binding to thyroid peroxidase (TPO). 125I-TPO binding by MoAb 47 was measured without or with decreasing concentrations (4 × 10−8–4 × 10−10 m) of Fab SP1.4, WR1.7, TR1.8 or TR1.9. The percentage inhibition is represented by a bar with shading corresponding to that in the inset. In the absence of competing Fab, binding by MoAb 47 was 14.3%.
