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The affinity of MoAb 47 for thyroid peroxidase (TPO) is lower than that of recombinant Fab. Unlike Fab TR1.9, 125I-TPO binding by MoAb 47 (and also by MoAb 15) is not inhibited by unlabelled TPO up to concentrations of 10−7 m. Data are shown as the mean ± range of duplicate determinations. Binding in the absence of unlabelled TPO was 15.9%, 15.2% and 13.6% for Fab TR1.9 (•), MoAb 47 (○) and MoAb 15 (▴), respectively.
