Table 2.
On day 21 supernatants were harvested for HIV-p24 titration by ELISA testing. Then the cultures were washed and refed with fresh medium containing the protein transport inhibitor brefeldin A. After 24 h incubation cells were fixed and stained for intracellular HIV-p24 and analysed by FACS. Five thousand to 10 000 cells were analysed for each sample. ND, Not done. The data are the arithmetic mean ± s.e.m. of three independent experiments.