Fig. 3.
Inhibition of IL-11 expression in fresh rheumatoid synovial cells (fresh RSC) by indomethacin and dexamethasone. (a) Fresh RSC were incubated in the presence or absence of 10−5 m indomethacin (IND) or 10−6 m dexamethasone (DEX) for 24 h. After incubation, the amounts of IL-11 in the supernatants were measured by ELISA. Each bar represents mean ± s.d. of three different wells. (b) Fresh RSC were incubated in the presence or absence of 10−5 m IND or 10−6m DEX for 6 h. After incubation, the treated cells were harvested for Northern blotting. Total RNA (10 μg) was size-fractionated, blotted, and hybridized with digoxigenin-labelled IL-11 cDNA as described in Materials and Methods. The lower part of the panel shows the 18S and 28S rRNA on ethidium bromide-stained gel as a loading control. Data are representative of three separate experiments.