Fig. 4.

Inhibitory effects of indomethacin and dexamethasone on the production of IL-11 by cultured rheumatoid synovial fibroblasts (RSF): the effects of prostaglandin E₂ (PGE₂). Rheumatoid synovial cells at four to eight passages were used as cultured RSF. The cells were cultured at a concentration of 5 × 10⁴/well in 24-well plates in the presence or absence of IL-1α (1 ng/ml) with or without 10⁻⁵ m indomethacin (IND) or 10⁻⁶ m dexamethasone (DEX) for 24 h. PGE₂ (10⁻⁵ m) was added in combination with IL-1α and IND, or IL-1α and DEX. The amounts of IL-11 in the supernatants were measured by ELISA. Each bar represents mean ± s.d. of three different wells.