Fig. 2.

Intracellular bromodeoxyuridine (BdU) staining of CD4⁺ and γδ⁺ T cell subsets responding to TBe (a,b); SN1 (c,d); and SN2 (e,f). Cells were stimulated with 9 μg/ml of each extract for 6 days and stained with MoAbs (PE-conjugated, ordinate) to CD4 (a,c,e) or γδ (b,d,f) T cell surface markers, followed by a MoAb (FITC-conjugated, abscissa) to BdU. Total CD4⁺ and γδ⁺ T cells were 44.6% and 1.16%, respectively, before culture (day 0). The percentage of BdU⁺ cells in the corresponding antigen-free culture (RPMI) was < 0.5% after 6 days of in vitro culture.