Fig. 6.

Responses of CD4⁺ and γδ⁺ T cell subsets to fractionated Mycobacterium tuberculosis extract (TBe). Concentrated TBe was fractionated on a Superose 12 HR column by fast performance liquid chromatography (FPLC). Fractions, as well as unfractionated extract, were tested in a 7-day proliferation assay and the results were expressed as absolute number of proliferating CD4⁺ (a) and γδ⁺ T cells (b), respectively. The column was calibrated with proteins of known molecular masses indicated above the graphics.