Fig. 3.

Different effect of yeasts and conidia of Penicillium marneffei on macrophage NO production. Macrophages (1 × 10⁶/well) were allowed to phagocytose the various numbers of opsonized yeasts or conidia of P. marneffei. After removing the unphagocytosed fungal elements, the cells were cultured with IFN-γ (300 U/ml) in the presence of amphotericin B (AmB; 0.05 μg/ml) for 48 h. The culture supernatants were collected and assayed for nitrite content. Experiments were repeated three times with similar results.