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IL-1β and tumour necrosis factor-alpha (TNF-α) secretion of cultured CD33+ cells. (a) IL-1β secretion of lamina propria mononuclear cells (LPMC) and sorted CD33+ cells from five different isolations. CD33+ cells secrete 4.2–40-fold more IL-1 than the LPMC. (b) TNF-α secretion after 24 h of culture in the medium of LPMC and the corresponding CD33-MACSbead-isolated colonic macrophages. Isolated CD33+ cells secreted between 4.5- and 44-fold more TNF-α.
