Fig. 1.

Immunoblotting of antigens in the cytosolic fraction of rat prostate separated in 15% SDS–PAGE under non-reducing conditions. Lanes 1 and 2 were incubated with normal sera diluted 1:50; lanes 3 and 4 were incubated with sera obtained after one immunization with modified rat accessory gland saline extract (MRAG)–Freund's complete adjuvant (FCA) diluted 1:50; lanes 5 and 6 were incubated with sera obtained after two immunizations with MRAG–FCA diluted 1:50. In each case two representative sera out of six are shown. The molecular weights of the markers from top to bottom are: 84, 63, 35, 32 and 21.5 kD.