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Immunoblotting of antigens in the cytosolic fraction of rat prostate (lane A), heart (lane B), liver (lane C) and lung (lane D) with or without treatment of the samples with 2-mercaptoethanol (2-ME). Normal serum (1) or autoimmune serum obtained after two immunizations (2) were used at a dilution of 1:50. The molecular weights of the markers from top to bottom are: 195, 112, 84, 63, 52, 35, 32 and 21.5 kD.
