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Western blot showing the reactivity of primary sclerosing cholangitis (PSC) sera with purified human α-enolase. α-enolase (1 μg/lane) was subjected to SDS–PAGE (4–20% polyacrylamide) and transferred to nitrocellulose sheets. Lane 1, prestained low molecular weight standard; lanes 2–6, Western blot with PSC sera (dilution 1:200) showing immunoreactivity against α-enolase in lanes 2–6.
