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Presence of human cytotoxic factor (hCF) in the culture supernatants (CS) of PBMC collected on different days after the onset of illness. ▪, ELISA; □, dot blot; ♦, cytotoxicity assay. The ‘cut off’ value was obtained from the culture supernatants of PBMC of 50 normal healthy individuals as mean value + 2 s.d. and was 25% for ELISA and 10% for the cytotoxicity assay. The figures in parentheses represent the total number of patients in each group.
