Fig. 4.

Enhancement of cytotoxicity of CD8⁺ cells by rIL-12 and/or rIL-2. CD8⁺ cells of the Sézary syndrome (SzS) patient at time point C were cultured in the presence of rIL-2 (50 U/ml), rIL-12 (1 or 10 ng/ml), rIL-12 (1 or 10 ng/ml) combined with rIL-2 (50 U/ml), rIFN-γ (1000 or 5000 U/ml), and rIFN-γ (1000 or 5000 U/ml) combined with rIL-2 (50 U/ml) with mitomycin-C- treated purified Sézary cells. Cytotoxicity of the cultured CD8⁺ cells (effector cells) was assayed by ⁵¹Cr-release of labelled Sézary cell (target cells) at an effector/target ratio of 10. Data are expressed as mean ± s.e.m. of the results of duplicate experiments.