Estimation of herpes virus DNA polymerase activity. Viral DNA polymerase activity was measured in transfected cell lines 2 h, 4 h, 6 h and 8 h post-infection (p.i.). Viral DNA polymerase activity was estimated using a commercially available ELISA system (see Materials and Methods). b, hatched bar, activity of Klenow enzyme in the presence of ammonium sulphate; ▪, neomycin-resistant cell line; hatched bars, exon 1′ La mRNA-expressing cell line; □, cell line expressing the human La gene. OD, Optical density. Error bars of two assays are given.