Fig. 1.

Comparison of allergic activities of wild-type rDer f 2 and C8/119S in A/J mice immunized with wild-type rDer f 2. (a) Passive cutaneous anaphylaxis (PCA) reaction provoked with wild-type rDer f 2 (□) and C8/119S (▪). Two rats intracutaneously sensitized with serial dilutions of hyperimmune plasma were challenged by either wild-type rDer f 2 or C8/119S. (b) IL-6 and IFN-γ production by spleen cells cultured for 72 h in the presence or absence of 10 μg/ml of either wild-type rDer f 2 or C8/119S. Spleen cells were prepared from three sensitized mice. Vertical bars represent s.e.m.