Fig. 2.

Western blots showing binding of autoantibodies from six patients to recombinant α(IV)NC1 domains. COS supernatants from transfected cells were separated on 12.5% SDS–PAGE gels and electroblotted to nitrocellulose. Recombinant protein was detected with human autoantibodies from Goodpasture patients A–F. Lane 1 contained collagenase-solubilized (cs) human glomerular basement membrane (GBM) (20 μg) as a positive control. The recombinant protein in the other lanes was: lane 2, human α3(IV)NC1; lane 3, human α1(IV)NC1; lane 4, human α5(IV)NC1.