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. 1998 Jul;113(1):17–27. doi: 10.1046/j.1365-2249.1998.00623.x

Fig. 4.

Fig. 4

Western blots showing binding of human autoantibodies from six patients to chimeric α3/α1(IV)NC1 domains. COS supernatants from transfected cells were separated on 12.5% SDS–PAGE gels and electroblotted to nitrocellulose. Recombinant chimeric human α1(IV)/α3(IV)NC1 domains were detected with sera from Goodpasture patients A–F. Lane 1 contained collagenase-solubilized (cs) human glomerular basement membrane (GBM) as a positive control. The recombinant protein in the other lanes was: lane 2, human α3(IV)NC1 (α333); lane 3, α113; lane 4, α131; lane 5, α311; lane 6, α331; lane 7, α313; lane 8, α133.