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ELISAs comparing binding of sera from patients with Goodpasture's disease to human collagenase-solubilized (cs) glomerular basement membrane (GBM), recombinant α3(IV)NC1 and recombinant α331. Three patients' sera are shown in assays performed in parallel on ELISA plates coated with (a) 4 μg/ml of cs-human GBM, (b) 1:2 dilution of purified recombinant antigen, or (c) purified recombinant α331. Antigen preparations were diluted in carbonate buffer pH 7.5.
