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ELISA showing the inhibition of human autoantibodies binding to human glomerular basement membrane (GBM) by human GBM or recombinant α3(IV)NC1 or recombinant α331. Doubling amounts (arbitrary units) of human or purified human recombinant antigen or recombinant α331 were preincubated with patients' autoantibodies. These were then allowed to bind to collagenase-solubilized (cs) human GBM on an ELISA plate. ▪, Human cs-GBM; •, recombinant antigen; ▴, recombinant α331.
