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. 1998 Jul;113(1):17–27. doi: 10.1046/j.1365-2249.1998.00623.x

Fig. 9.

Fig. 9

Western blot and autoradiograph of radiolabelled chimeric human α3(IV)/α1(IV)NC1 domains. COS supernatants from transfected cells were separated on 12.5% SDS–PAGE gels, which were then electroblotted to nitrocellulose or dried for exposure to autoradiograph film. (a) Western blot showing binding of Goodpasture autoantibodies to radiolabelled chimeric α3/α1(IV)NC1 domains. (b) Autoradiograph showing the expression levels of chimeric human α3(IV)/α1(IV)NC1 domains. The radiolabelled recombinant protein in each lane was: lane 1, human α1(IV)NC1 (α111); lane 2, α113; lane 3, α131; lane 4, α311; lane 5, α331; lane 6, α313; lane 7, α133; lane 8, human α3(IV)NC1 (α333).