Fig. 2.

Kinetics of nitrite production by LPS-activated J774 macrophages. J774 cells were activated with LPS (1 μg/ml) in the absence or presence of rIL-10 (10 U/ml) and nitrite levels were determined in supernatants collected at the indicated time points. Data are shown as mean ± s.d. of three independent experiments performed in triplicate. P = 0.01, 0.02 and 0.04 after 16, 24 and 48 h, respectively, for macrophages treated with LPS and IL-10 compared with macrophages treated with LPS alone (Student's t-test).