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Immunoprecipitation of baculovirus-expressed human IA-2 with insulin-dependent diabetes mellitus (IDDM) serum. Sf-9 cells were labelled with 35S-methionine 48 h after infection with Bac-hIA-2 recombinant virus. Various amounts of labelled cell lysates (lanes 1 and 4, 40 000 ct/min; lanes 2 and 5, 80 000 ct/min; and lanes 3 and 6, 60 000 ct/min) were immunoprecipitated either with IDDM sera (lanes 1–3) or control sera (lanes 4–6) and then subjected to 10% SDS–PAGE.
