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Pattern of expression of all tetraspan transmembrane antigen (TST) antigens in several cell lines (KK124, Ly67, Jijoye and BL28) to illustrate the detection of the major common pattern, the presence of strong signal for CD53, CD81 and CD63. As starting material we used 25–35 ng of total RNA for the synthesis of the cDNA which was utilized as target for 30 cycles of polymerase chain reaction (PCR) amplification with gene-specific oligonucleotides.
