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Cell surface expression of DAF on HT-29 cells and the effect of phosphatidylinositol-specific phospholipase C (PIPLC) treatment. Cells were detached, incubated with 1C6 anti-DAF antibody (a) or an irrelevant MoAb (c), then with an FITC-conjugated anti-mouse IgG, and analysed by flow cytometry. Cells treated with PIPLC (100 mU/ml) were also examined as described above (b). This experiment was repeated twice with similar results.
