Fig. 4.

Recovery of DAF in the fractions separated by centrifugation of the culture supernatant of HT-29 cells. Cells were incubated for 24 h with or without addition of IL-1β (10 ng/ml) or IL-4 (100 ng/ml). The culture supernatant was centrifuged at 15 000 g for 15 min, and its supernatant was further centrifuged at 100 000 g for 60 min. DAF in each fraction was measured by use of ELISA. Values are expressed as the amount of DAF in 1 ml of the culture supernatant.