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Serum immunoglobulin levels in mercury-treated IL-4+ (▪) and IL-4−/− mice (□). Quantification of immunoglobulin isotypes was performed using a sandwich ELISA as described previously [14]. Eight mice were included in the IL-4+ group and four mice in the IL-4−/− group. Comparisons between the two groups were performed using the Mann–Whitney test. *Statistically significant (P < 0.05) differences.
