Fig. 1.

Mice AB2 titres after immunization with anti-gp43 MoAb 17c and idiotope specificity of sera. (a) Binding of ¹²⁵I-gp43 to anti-gp43 MoAb 17c-coated plates performed in the presence of diluted sera from a representative immunized mouse with anti-gp43 MoAb 17c, as indicated in Materials and Methods. (b) Binding of ¹²⁵I-gp43 to anti-gp43 MoAb 17c-coated plates performed in the presence of diluted sera from immunized mice with anti-gp43 MoAb 24a and 8a. No relevant inhibition of binding was observed when mice sera immunized with the latter MoAbs were added. Assays were made in triplicates.