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Peripheral blood mononuclear cells (PBMC) from five normal controls (N) were cultured with hsp65 or hsp65 with IL-6 plus IFN-γ, IL-6 plus IL-2 or TNF-α plus IFN-γ in the presence or absence of anti-IFN-γ (0.4 μg/ml) or anti-TNF-α (30 ng/ml) antibodies during 7 days. Then cultured mononuclear cells were tested for their cytotoxic activity and results are expressed as percentage of cytotoxicity.
