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High performance liquid chromatography (HPLC) analysis of pooled human immunoglobulin (PHIG)-treated Streptococcus pyrogenic exotoxin A (SPE-A). SPE-A (100 ng/ml) was incubated with or without 5 mg/ml PHIG for 2 h. Then, buffer-diluted SPE-A (a), SPE-A + PHIG (b) and PHIG (c) were subjected to a gel permeation HPLC and areas of SPE-A and PHIG peaks were compared.
