Fig. 2.

Isolation of N185 peptide-specific, DRB1*1103-restricted T cell clones. T cells (3 × 10⁴) were added to γ-irradiated B cells (2 × 10⁴) with the indicated antigen. After 2 days, 1 μCi ³H-thymidine was added for the last 18 h of culture and incorporated radioactivity was determined. (a) Proliferation of N5 T cells was measured following stimulation by uninfected 6396 B cells (−), measles virus (MV)-infected 6396 B cells (MV), recombinant vaccinia virus containing the nucleoprotein gene (vv-N)-infected 6396 B cells (vv-N), recombinant vaccinia virus carrying the MV fusion protein (vv-F)-infected 6396 B cells (vv-F) and uninfected 6396 B cells in the presence of 1 μg cyanogen bromide (CNBr)-digested nucleoprotein (peptides). (b) Proliferation of N5 T cells was measured following stimulation by 6396 B cells and graded concentrations of N185 or bN185 peptide. (c) Proliferation of N5 T cells was measured following stimulation by uninfected 6396 B cells (−) or MV-infected 6396 B cells in the absence (MV/-) or the presence of anti-DR MoAb L.243 (MV/L.243), anti-DP MoAb B7/21 (MV/B7/21) or anti-DQ MoAb SVPL.3 (MV/SVPL.3). (d) Proliferation of N5 and N2 T cells was measured following stimulation by various MV-infected B cell lines (6396: DR1,DRw11; 11.3: DRB1*1101,DR3; JVM: DRB1*1102 homozygous; 4036: DRB1*1103,DR4; 4029: DRB1*1102,DRB1*1104; KS: DRB1*1104,DR6; and LG-2: DRB1*0101 homozygous). Values are means of duplicates. Variations were < 15% of the mean.