Fig. 2.

Reactivity of S1 against liver cytosol and three enzymes from a panel of 18 (see Table 2). Well 1 contains undiluted S1, wells A, C, E liver cytosol (the source of the anti-liver cytosol type 1 (LC1) antigen) at 4 mg/ml, well B glutathione peroxidase (4 mg/ml), well D lactic dehydrogenase (4 mg/ml), well F argininosuccinate lyase (ASL) 4 mg/ml. A precipitin line is formed against bovine ASL (F), but not against glutathione peroxidase (B) or lactic dehydrogenase (D). The line against the cytosolic fraction is broad, suggesting the possibility that it may contain two antigens, particularly so between wells 1 and A.