Fig. 7.

Activation state of lymphocytes from NOD mice after mycobacterial infection. NOD mice were intraperitoneally infected with 10⁸ Mycobacterium avium bacilli or injected with the vehicle of the bacteria. Cell acquisition was performed gating each subset of lymphocytes (CD3⁺, CD4⁺, CD8⁺, IgM⁺, IgG⁺) and the percentage of CD69⁺ cells was determined. The number of CD69⁺ lymphocytes was derived from the percentage of CD69⁺ cells and the numbers of splenic CD3⁺, CD4⁺, CD8⁺, IgM⁺ and IgG⁺ lymphocytes. Mycobacterial infection induced significant increases in the numbers of activated, CD69-expressing T cells (both CD4⁺ and CD8⁺) and activated CD69⁺ B cells (expressing either surface IgM or IgG). 1,0E + 08 = 10⁸. n = 8. □, Control; ▪, M. avium.