Fig. 5.

Effects of cyclophosphamide (CY) on islet T cell pathogenicity and cytokine production. (a) Two-month-old male NOD mice were treated with CY (▪) or with PBS (○), their islet lymphocytes were transferred into groups of 5–10 male NOD.scid mice, and blood glucose was monitored. *P < 0.015. (b) Islet lymphocytes were isolated from CY-treated or PBS-treated normoglycaemic NOD males and analysed for the frequencies of IFN-γ- (▪) and IL-4-secreting T cells (□) by the ELISPOT assay. The mean SFC of duplicate wells are shown from one of two similar experiments. *P < 0.05 compared with PBS-treated controls. (c) Male NOD.scid mice, five per group, were injected with male NOD islet lymphocytes and treated either with CY (▪) (n = 5) or PBS (○) (n = 10). The development of diabetes was monitored. *P < 0.003.