Fig. 2.

IgG reactivity to nitrocellulose-blotted human epithelial (a), endothelial (b), myocard (c) and skeletal muscle (d) protein extracts. Epithelial, endothelial, and skeletal muscle extracts were exposed to sera from cardiomyopathy (CMP) patients (children/juveniles, lanes 1–28; adults, lanes 1–8) and from healthy individuals (lanes 1–9). Myocard extracts (c) were probed with the same sera and in addition with nine sera from patients with coronary heart disease and one additional healthy person. Lanes B represent the buffer control without addition of serum. Molecular weights are displayed in kD. The large arrow in (c) indicates the position of anti-myosin immunoreactivity.