Fig. 2.

Cytokine mRNA profile of unstimulated peripheral blood mononuclear cells (PBMC) reveals decreased production of IFN-γ, production of IL-4, and increased production of IL-10 in AD patient compared with healthy donor. Cytokine gene expression in PBMC of an AD patient (a,b) and a healthy subject (c,d), unstimulated (a,c) and stimulated for 6 h with phorbol myristate acetate (PMA) and ionomycin (b,d). Reverse transcriptase-polymerase chain reaction (RT-PCR) products were analysed on a 15% agarose gel and detected by ethidium bromide staining. The molecular weight marker used is φX174 DNA HaeIII digest.