Fig. 1.

(a) SDS–PAGE analysis of the ATP binding protein from Cryptococcus neoformans whole cell extract. The antigens were electrophoresed on a 10% gel and then stained with silver. Positions of molecular mass markers are shown on the left. (b) Western blot analysis of ATP binding protein including hsp70 from C. neoformans with sera from patients with pulmonary cryptococcosis. The antigens were electrophoresed on a 10% gel, transferred to PVDF membrane, and incubated with a 1:50 dilution of sera from patients with pulmonary cryptococcosis or 1:250 dilution of sera from infected mice. Lanes A–U, sera from patients with pulmonary cryptococcosis; lane X, sera from infected mice (post-infection day 23). The positions of protein molecular weight markers, with respective sizes, are indicated on the left.