Fig. 2.

Failure of anti-IL-10 and anti-TGF-β antibodies to modulate macrophage anti-Mycobacterium avium-intracellulare complex (MAIC) activity. The result shown is representative of six experiments separately carried out. Peritoneal macrophages of BALB/c (Bcg^S) mice were infected with high-virulence MAIC N-260 strain and cultured in the absence (□) or presence of 30 μg/ml each of anti-IL-10 antibody, anti-TGF-β antibody, or control antibody (rat IgG for anti-IL-10 antibody and mouse IgG for anti-TGF-β antibody). *Smaller than the value of control macrophages (P < 0.05; Mann–Whitney test). There was no significant difference in the bacterial loads between macrophages treated with each anti-cytokine antibody and those treated with a control antibody. The other details are the same as in Fig. 1.