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. 1999 Jun;116(3):449–452. doi: 10.1046/j.1365-2249.1999.00820.x

Fig. 2.

Fig. 2

Dot plot analysis of decidual lymphocytes. FL1 (CD16) and FL2 (CD56) were gated with CD3− cells. A large number of CD56+16−3− cells of missed abortions with abnormal chromosomes are found in the left panel. Decreased number of CD56+16−3− NK cells in missed abortions with normal chromosomes are found in the right panel. A large proportion of the NK cells in this group were still CD56+ (CD56+dim), but they were less bright than in the group with abnormal chromosomes. The lymphocytes were isolated from the decidua and stained with FITC–anti-CD16, PE–anti-CD56 and PerCP–anti-CD3 MoAb. The first gate was set around the lymphocytes. The second gate was set around CD3− lymphocytes. Ten thousand lymphocytes were analysed on a FACS Caliber. Results with all antibodies were expressed as the percentage of these gated cells. CD56+dim cells were counted as CD56+.